Diagnostic Kit for C-reactive Protein (Immunochromatographic assay)
Ⅰ.Test principle
Diagnostic Kit for C-reactive Protein is used the principle of
fluorescence immunochromatography. The CRP antigen in the sample
first combines with the fluorescent labeled CRP monoclonal antibody
conjugate, and then continues to move and combine with another CRP
monoclonal antibody fixed on the nitrocellulose membrane to form a
double antibody sandwich immune complex at the detection line of
nitrocellulose membrane.
Ⅱ.Product Description
| Name | Loading capacity | Component |
| Test card | 25/40 | It is composed of fluorescent pad (coated with fluorescent labeled
CRP monoclonal antibody), nitrocellulose membrane (coated with CRP
monoclonal antibody and Goat anti mouse IgG antibody), absorbent
paper and backing. |
| Sample diluent | 25/40 | Phosphate buffer |
| ID card | 1 | Record the standard curve information of this batch of reagents |
The components in different batches of kits cannot be used
interchangeably.
Intended use:Diagnostic Kit for C-reactive Protein is used for
quantitative detection of C-reactive protein (CRP) in human serum,
plasma and whole blood. CRP is mainly used as a non-specific
inflammatory index.
Ⅲ.Using the step
1. Before the test, please read the instructions completely. If
Diagnostic Kit for C-reactive Protein test card and sample are
stored in cold storage, they should be balanced at room temperature
(15-30)℃ for not less than 30min before use.
2. Start NIR-1000 dry fluoroimmunoassay analyser and correctly
select the corresponding sample type on the instrument.
3. Take out the ID card, make sure that the batch number of the ID
card is consistent with that of the test card, and insert the ID
card into the ID card port of the instrument.
4. Take out the test card from the aluminum foil bag and use it
within 15 minutes.
5. Place the test card on a clean horizontal table and mark it
horizontally.
6. Mix 5 µL of sample with 1000µL of sample diluent. Apply 100 µL
of diluted samples to the well of the test card.
7. At 3 minutes after addition of samples, insert the test card
into NIR-1000 dry fluoroimmunoassay analyser and click the “Instant
test” button to read the results.
Ⅳ.Sample requirements
1. Serum and EDTA•Na2 anticoagulant plasma and whole blood, EDTA•K2
anticoagulant plasma and whole blood, sodium citrate anticoagulant
plasma and whole blood can be used.
2. Venous blood was collected according to routine laboratory
methods, and hemolysis was avoided as much as possible in the
process of treatment.
3. After clinical samples were collected, the detection was
completed within 4 hours at room temperature (15-30) ℃.The whole
blood sample can be stored for 24 hours at (2-8)℃ without freezing;
Serum and plasma samples can be stored for 7 days at 2-8℃;Serum and
plasma samples -20℃ for 30 days.
4. The sample must return to room temperature (15-30)℃ before
testing. The frozen samples should be completely thawed, rewarming
and mixed evenly before use, and repeated freezing and thawing
should be avoided.
5. Do not test samples with severe hemolysis, severe lipidemia and
jaundice.
Ⅴ.CRP-hsCRP.pdf
