Diagnostic Kit for Serum Ferritin(Immunochromatographic assay)
Ⅰ.Product Description
| Name | Quantity | Component |
| Test card | 25/40 | It is composed of fluorescent pad (coated with fluorescent labeled
Ferr monoclonal antibody), nitrocellulose membrane (coated with
Ferr monoclonal antibody and Goat anti mouse IgG antibody),
absorbent paper and backing |
| Sample buffer | 25/40 | Phosphate buffer |
| ID card | 1 | Record the standard curve information of this kit |
The components in different batches of kits cannot be used
interchangeably.
Package specification:25Tests/kit,40 Tests/kit
Intended use:Diagnostic Kit for Serum Ferritin is used to quantitatively detect
the content of ferritin in human serum. Clinically, it is mainly
used for the auxiliary diagnosis of iron metabolism related
diseases, such as hemochromatosis and iron deficiency anemia.
Test principle:The principle of immunofluorescence chromatography was applied to
the Diagnostic Kit for Serum Ferritin. The Ferr antigen in the
sample was first bound with the conjugated compound of fluorescent
labeled Ferr monoclonal antibody, then moved and combined with
another Ferr monoclonal antibody fixed on the nitrocellulose
membrane, and the double antibody sandwich complex was formed at
the detection line of the cellulose nitrate membrane.
Ⅱ.Using the step
1. Before the test, please read the instructions completely. If the
test card and sample are stored in cold storage, they should be
balanced at room temperature (15-30)℃ for not less than 30min
before use.
2. Start NIR-1000 dry fluoroimmunoassay analyser and correctly
select the corresponding sample type on the instrument.
3. Take out the ID card, make sure that the batch number of the ID
card is consistent with that of the test card, and insert the ID
card into the ID card port of the instrument.
4. Take out the test card from the aluminum foil bag and use it
within 15 minutes.
5. Place the test card on a clean horizontal table and mark it
horizontally.
6. Mix 20 µL of sample with 400µL of sample diluent. Apply 100 µL
of diluted samples to the well of the test card.
7. At 5 minutes after addition of samples, insert the test card
into NIR-1000 dry fluoroimmunoassay analyser and click the “Instant
test” button to read the results.
Ⅲ.Reference interval
Through the determination of serum samples from 301 healthy people
aged 10 ~ 87, the results were statistically analyzed.
| Gender | 2.5th percentile (ng/mL) | 97.5th percentile (ng/mL) |
| Male | 24 | 335 |
| Female | 11 | 307 |
Ⅳ.Why to choose us?
1. Analysis sensitivity
No higher than 5ng/mL.
2. Accuracy
The relative deviation from the target value is within ±15%.
3. Precision
The within and between assay coefficient of variations are within
15%.
4. Linear range
Within the linear range (5 ~ 500ng/mL), the linear correlation
coefficient R≥0.990
Ⅴ.FERR.pdf
