【Product name】 19.
IL-6.pdf
Diagnostic Kit for Interleukin-6(Immunochromatographic assay)
【Intended use】
The kit is used for quantitative determination of Interleukin-6
(IL-6) in human whole blood, plasma and serum.
【Test principle】
The kit is a one-step chromatographic sandwich immunoassay designed
for the quantitative measurement of IL-6. The IL-6 antigen in the
sample was first bound with the conjugated compound of fluorescent
labeled IL-6 monoclonal antibody, then moved and combined with
another IL-6 monoclonal antibody fixed on the nitrocellulose
membrane, and the double antibody sandwich complex was formed at
the detection line of the cellulose nitrate membrane.
【Applicable instrument】
NIR-1000 dry fluoroimmunoassay analyser produced by WWHS Biotech.
Inc.
【Test procedure】
- Before the test, please read the instructions completely. If the
test card and sample are stored in cold storage, they should be
balanced at room temperature (15-30)℃ for not less than 30min
before use.
- Start NIR-1000 dry fluoroimmunoassay analyser according to the
instruction manual of the instrument, and carry out quality control
verification according to the instruction manual of the
instrument(Note: the reagent has been calibrated in advance, and
the calibration curve parameters of each batch of reagent have been
stored in the ID card. The test can be carried out only after the
quality control is passed. Otherwise, the cause should be found out
before testing.)
- Remove the test card from the aluminum foil bag and use it within
15 minutes.
- Place the test card on a clean horizontal table and mark it
horizontally.
- Mix 100 µL of patient sample with 300µL of sample diluent. Apply
100 µL of diluted samples to the well of the test card.
- Insert the test card into NIR-1000 dry fluoroimmunoassay analyser,
read and record the results at 10 minutes after addition of
samples, then dispose of used test appropriately.
【Note】
1. The kit can be used for in vitro diagnosis only.
2. Test card and buffer solution are single-use and they cannot be
reused.
3. Please check the integrity and validity of the kit package
before use, and then open the package. When it is stored at low
temperature, it should be restored to room temperature (15℃ ~ 30℃)
before opening the package for use. The reagents with damaged inner
package and beyond the validity period cannot be used.
4. Take the test card out of the aluminum foil bag and carry out
experiment in 15min. Do not place it in the air for a long time to
avoid dampness.
5. It is required to strictly comply with the requirements for
sample collection and storage. If the sample is turbid, please
centrifuge and precipitate it before use.
6. The kit used should be disposed of as latent infective material,
and all samples, reagents and latent contaminants should be
disinfected and disposed of according to relevant local
regulations.
| WWHS Assay list | Inflammation | | | | |
| cat#. | Product Item | Specimen | Reaction Time | Measure Range | Clinical Range | Intended Use |
| 10 | CRP / hs-CRP | WB/Serum/Plasma | 3min. | 0.5-200mg/L | CRP<10mg/L,hs-CRP<1mg/L | nonspecficity inflammatory marker. |
| 11 | SAA | Serum | 5min. | 1-200mg/L | <10mg/L | inflammation&infection. |
| 12 | PCT | WB/Serum/Plasma | 10min. | 0.2-100ng/ml | <0.5ng/ml | Sepsis |
| 13 | CRP+SAA | WB/Serum/Plasma | 5min. | same with single item | same with single item | inflammation&infection. |
| 14 | IL-6 | WB/Serum/Plasma | 10min. | 5-4000pg/ml | 10pg/ml | diabetes,rheumatoid arthritis,etc |
